MEK2 ELISA 试剂盒
MAP2K2
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all MEK2 (MAP2K2) ELISA试剂盒
- MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human MEK2/MAP2K2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human MEK2.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product MAP2K2 ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all MEK2 (MAP2K2) ELISA试剂盒
- MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))
- 别名
- MEK2 (MAP2K2 (MAP2K2 产品)
- 别名
- CFC4 ELISA Kit, MAPKK2 ELISA Kit, MEK2 ELISA Kit, MKK2 ELISA Kit, PRKMK2 ELISA Kit, AA589381 ELISA Kit, MK2 ELISA Kit, Prkmk2 ELISA Kit, ATMKK2 ELISA Kit, F27B13.50 ELISA Kit, F27B13_50 ELISA Kit, MAP KINASE KINASE 1 ELISA Kit, MAP kinase kinase 2 ELISA Kit, MK1 ELISA Kit, map2k2 ELISA Kit, wu:fa56c06 ELISA Kit, zgc:123171 ELISA Kit, zgc:172250 ELISA Kit, mitogen-activated protein kinase kinase 2 ELISA Kit, mitogen activated protein kinase kinase 2 ELISA Kit, MAP kinase kinase 2 ELISA Kit, mitogen-activated protein kinase kinase 2a ELISA Kit, mitogen-activated protein kinase kinase 2b ELISA Kit, MAP2K2 ELISA Kit, Map2k2 ELISA Kit, MKK2 ELISA Kit, map2k2a ELISA Kit, map2k2b ELISA Kit
- UniProt
- P36507
- 途径
- MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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