FGFR2 ELISA 试剂盒
FGFR2
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all FGFR2 ELISA试剂盒
- FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse FGFR2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse FGFR2.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product FGFR2 ELISA Kit
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Fibroblast Growth Factor Receptor 2 (FGFR2) ELISA Kit
FGFR2 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all FGFR2 ELISA试剂盒
- FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))
- 别名
- FGFR2 (FGFR2 产品)
- 别名
- FGFR2 ELISA Kit, FGFR2IIIb ELISA Kit, bek ELISA Kit, bfr-1 ELISA Kit, cd332 ELISA Kit, cek3 ELISA Kit, cfd1 ELISA Kit, ect1 ELISA Kit, fgfr-2 ELISA Kit, jws ELISA Kit, k-sam ELISA Kit, kgfr ELISA Kit, tk14 ELISA Kit, tk25 ELISA Kit, xfgfr2 ELISA Kit, DKFZp469H1521 ELISA Kit, BBDS ELISA Kit, BEK ELISA Kit, BFR-1 ELISA Kit, CD332 ELISA Kit, CEK3 ELISA Kit, CFD1 ELISA Kit, ECT1 ELISA Kit, JWS ELISA Kit, K-SAM ELISA Kit, KGFR ELISA Kit, TK14 ELISA Kit, TK25 ELISA Kit, AU043015 ELISA Kit, AW556123 ELISA Kit, Bek ELISA Kit, Fgfr-2 ELISA Kit, Fgfr-7 ELISA Kit, Fgfr7 ELISA Kit, KGFRTr ELISA Kit, svs ELISA Kit, FGF-R ELISA Kit, fgfr72IIIb ELISA Kit, FGFR ELISA Kit, fc56c05 ELISA Kit, wu:fc56c05 ELISA Kit, fibroblast growth factor receptor 2 ELISA Kit, fibroblast growth factor receptor 2 L homeolog ELISA Kit, FGFR2 ELISA Kit, LOC100084641 ELISA Kit, fgfr2 ELISA Kit, Fgfr2 ELISA Kit, fgfr2.L ELISA Kit
- UniProt
- P21803
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Growth Factor Binding
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