ECE1 ELISA 试剂盒
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- 抗原 See all ECE1 ELISA试剂盒
- ECE1 (Endothelin-Converting Enzyme 1 (ECE1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ECE-1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human ECE-1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product ECE1 ELISA Kit
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Endothelin-Converting Enzyme 1 (ECE1) ELISA Kit
ECE1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Endothelin-Converting Enzyme 1 (ECE1) ELISA KitECE1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all ECE1 ELISA试剂盒
- ECE1 (Endothelin-Converting Enzyme 1 (ECE1))
- 别名
- ECE-1 (ECE1 产品)
- 别名
- ECE ELISA Kit, Ece ELISA Kit, AW322500 ELISA Kit, ECE-1 ELISA Kit, ECE-1a ELISA Kit, ECE-1b ELISA Kit, ECE-1d ELISA Kit, ece1 ELISA Kit, MGC52874 ELISA Kit, MGC130791 ELISA Kit, fd11b07 ELISA Kit, wu:fd11b07 ELISA Kit, zgc:154079 ELISA Kit, endothelin converting enzyme 1 ELISA Kit, endothelin converting enzyme like 1 ELISA Kit, endothelin converting enzyme 1 L homeolog ELISA Kit, endothelin-converting enzyme 1 ELISA Kit, Endothelin-converting enzyme 1 ELISA Kit, ECE1 ELISA Kit, ECEL1 ELISA Kit, Ece1 ELISA Kit, ece1.L ELISA Kit, Za10_0551 ELISA Kit, Arch_0474 ELISA Kit, Closa_0961 ELISA Kit, Odosp_0578 ELISA Kit, Bacsa_1787 ELISA Kit, Weevi_2017 ELISA Kit, Fluta_3444 ELISA Kit, Sphch_2390 ELISA Kit, ece1 ELISA Kit
- UniProt
- P42892
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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