DLK1 ELISA 试剂盒
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- 抗原 See all DLK1 ELISA试剂盒
- DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 4.5 pg/mL - 1000 pg/mL
- 最低检测浓度
- 4.5 pg/mL
- 应用范围
- ELISA
- 原理
- Mouse Pref-1/DLK-1/FA1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Pref-1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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delta-Like 1 Homolog (Drosophila) (DLK1) ELISA Kit
DLK1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
delta-Like 1 Homolog (Drosophila) (DLK1) ELISA KitDLK1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
delta-Like 1 Homolog (Drosophila) (DLK1) ELISA KitDLK1 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 试剂准备
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Recommended Dilution for serum and plasma samples25 fold
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all DLK1 ELISA试剂盒
- DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))
- 别名
- Pref-1 (DLK-1/FA1) (DLK1 产品)
- 别名
- DELTA1 ELISA Kit, DL1 ELISA Kit, Delta ELISA Kit, DLK ELISA Kit, DLK-1 ELISA Kit, Delta1 ELISA Kit, FA1 ELISA Kit, PREF1 ELISA Kit, Pref-1 ELISA Kit, ZOG ELISA Kit, pG2 ELISA Kit, AW742678 ELISA Kit, DlkI ELISA Kit, Ly107 ELISA Kit, Peg9 ELISA Kit, SCP1 ELISA Kit, pref-1 ELISA Kit, Zog ELISA Kit, delta like canonical Notch ligand 1 ELISA Kit, delta like non-canonical Notch ligand 1 ELISA Kit, delta-like 1 homolog (Drosophila) ELISA Kit, delta-like 1 (Drosophila) ELISA Kit, DLL1 ELISA Kit, DLK1 ELISA Kit, Dll1 ELISA Kit, Dlk1 ELISA Kit
- UniProt
- Q09163
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