CPNE1 ELISA 试剂盒
CPNE1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all CPNE1 products
- CPNE1 (Copine I (CPNE1))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Copine-1/CPNE1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Copine-1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CPNE1 products
- CPNE1 (Copine I (CPNE1))
- 别名
- Copine-1 (CPNE1 (CPNE1 产品)
- 别名
- CPNE1 ELISA Kit, MGC108079 ELISA Kit, COPN1 ELISA Kit, CPN1 ELISA Kit, 1810028N16Rik ELISA Kit, mKIAA4108 ELISA Kit, cpne3 ELISA Kit, cpne3l ELISA Kit, wu:fb69d07 ELISA Kit, wu:fc45a04 ELISA Kit, zgc:55873 ELISA Kit, copine I ELISA Kit, copine 1 ELISA Kit, RNA-binding protein 12 ELISA Kit, copine-1 ELISA Kit, copine I L homeolog ELISA Kit, CPNE1 ELISA Kit, cpne1 ELISA Kit, CPNE1b ELISA Kit, LOC100032499 ELISA Kit, LOC100484845 ELISA Kit, EDI_033120 ELISA Kit, LOC100281056 ELISA Kit, cpne1.L ELISA Kit, Cpne1 ELISA Kit
- UniProt
- Q99829
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