CD45 ELISA 试剂盒
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- 抗原 See all CD45 (PTPRC) ELISA试剂盒
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.26 ng/mL - 15 ng/mL
- 最低检测浓度
- 0.26 ng/mL
- 应用范围
- ELISA
- 原理
- Mouse CD45 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse CD45.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product PTPRC ELISA Kit
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Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) ELISA Kit
PTPRC 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) ELISA KitPTPRC 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) ELISA KitPTPRC 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) ELISA KitPTPRC 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 试剂准备
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Recommended Dilution for serum and plasma samples2 fold
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CD45 (PTPRC) ELISA试剂盒
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- 别名
- CD45 (PTPRC 产品)
- 别名
- PTPRC ELISA Kit, si:dkeyp-47c5.1 ELISA Kit, B220 ELISA Kit, CD45 ELISA Kit, CD45R ELISA Kit, GP180 ELISA Kit, L-CA ELISA Kit, LCA ELISA Kit, LY5 ELISA Kit, T200 ELISA Kit, Cd45 ELISA Kit, Ly-5 ELISA Kit, Lyt-4 ELISA Kit, loc ELISA Kit, Lca ELISA Kit, RT7 ELISA Kit, cd45 ELISA Kit, protein tyrosine phosphatase, receptor type C ELISA Kit, protein tyrosine phosphatase, receptor type, C ELISA Kit, receptor-type tyrosine-protein phosphatase C ELISA Kit, PTPRC ELISA Kit, ptprc ELISA Kit, LOC100074271 ELISA Kit, Ptprc ELISA Kit
- UniProt
- P06800
- 途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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