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METAP2 ELISA 试剂盒
METAP2
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
METAP2ELISA Kit详情
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METAP2
(Methionyl Aminopeptidase 2 (METAP2))
适用
All reactivities for METAP2 ELISA试剂盒
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Mouse METAP2 ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes Mouse METAP2.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
METAP2目标详情
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METAP2
(Methionyl Aminopeptidase 2 (METAP2))
别名
METAP2 (METAP2 产品 )
别名
metap2 ELISA Kit, MGC53792 ELISA Kit, METAP2 ELISA Kit, GB13458 ELISA Kit, DDBDRAFT_0190923 ELISA Kit, DDBDRAFT_0304991 ELISA Kit, DDB_0190923 ELISA Kit, DDB_0304991 ELISA Kit, 4930584B20Rik ELISA Kit, A930035J23Rik ELISA Kit, AI047573 ELISA Kit, AL024412 ELISA Kit, AU014659 ELISA Kit, Amp2 ELISA Kit, Mnpep ELISA Kit, p67 ELISA Kit, p67eIF2 ELISA Kit, MAP2 ELISA Kit, MNPEP ELISA Kit, wu:fb98h06 ELISA Kit, zgc:66250 ELISA Kit, methionyl aminopeptidase 2 L homeolog ELISA Kit, methionyl aminopeptidase 2 ELISA Kit, methionine aminopeptidase 2 ELISA Kit, methionine aminopeptidase ELISA Kit, methionyl aminopeptidase 2 S homeolog ELISA Kit, methionyl aminopeptidase 2b ELISA Kit, metap2.L ELISA Kit, METAP2 ELISA Kit, metap2 ELISA Kit, LOC551771 ELISA Kit, CAALFM_C604080WA ELISA Kit, Metap2 ELISA Kit, metap2.S ELISA Kit, metap2b ELISA Kit
UniProt
O08663
途径
Regulation of G-Protein Coupled Receptor Protein Signaling
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