PLA2G1B ELISA 试剂盒
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- 抗原 See all PLA2G1B ELISA试剂盒
- PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse PLA2G1B ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse PLA2G1B.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling PLA2G1B ELISA Kit
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- Discover our top product PLA2G1B ELISA Kit
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Phospholipase A2, Group IB (PLA2G1B) ELISA Kit
PLA2G1B 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IB (PLA2G1B) ELISA KitPLA2G1B 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Phospholipase A2, Group IB (PLA2G1B) ELISA KitPLA2G1B 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all PLA2G1B ELISA试剂盒
- PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))
- 别名
- PLA2G1B (PLA2G1B 产品)
- 别名
- C133 ELISA Kit, CG4346 ELISA Kit, Dmel\\CG42237 ELISA Kit, Dmel_CG4346 ELISA Kit, PLA2 ELISA Kit, GB13351 ELISA Kit, bvPLA2 ELISA Kit, PLA2A ELISA Kit, PPLA2 ELISA Kit, sPLA(2)-IB ELISA Kit, Pla2a ELISA Kit, sPLA2IB ELISA Kit, PLA2G1B ELISA Kit, CG42237 gene product from transcript CG42237-RB ELISA Kit, phospholipase A2 ELISA Kit, phospholipase A2 group IB ELISA Kit, phospholipase A2, group IB, pancreas ELISA Kit, phospholipase A2 group IVA ELISA Kit, CG42237 ELISA Kit, PLA2 ELISA Kit, LOC100631065 ELISA Kit, Pla2 ELISA Kit, PLA2G1B ELISA Kit, Pla2g1b ELISA Kit, PLA2G4A ELISA Kit
- UniProt
- Q9Z0Y2
- 途径
- Inositol Metabolic Process, VEGF Signaling
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