电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

IGSF8 ELISA 试剂盒

IGSF8 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6730309
发货至: 中国
  • 抗原 See all IGSF8 ELISA试剂盒
    IGSF8 (Immunoglobulin Superfamily, Member 8 (IGSF8))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human IGSF8 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human IGSF8.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all IGSF8 ELISA试剂盒
    IGSF8 (Immunoglobulin Superfamily, Member 8 (IGSF8))
    别名
    IGSF8 (IGSF8 产品)
    别名
    MGC77222 ELISA Kit, IGSF8 ELISA Kit, MGC151661 ELISA Kit, CD316 ELISA Kit, CD81P3 ELISA Kit, EWI-2 ELISA Kit, EWI2 ELISA Kit, KCT-4 ELISA Kit, LIR-D1 ELISA Kit, PGRL ELISA Kit, AA033172 ELISA Kit, AU041109 ELISA Kit, ESTM34 ELISA Kit, R75384 ELISA Kit, immunoglobulin superfamily, member 8 ELISA Kit, immunoglobulin superfamily member 8 ELISA Kit, igsf8 ELISA Kit, IGSF8 ELISA Kit, Igsf8 ELISA Kit
    UniProt
    Q969P0
You are here:
客服