S100A1 ELISA 试剂盒
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- 抗原 See all S100A1 ELISA试剂盒
- S100A1 (S100 Calcium Binding Protein A1 (S100A1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse S100A1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse S100A1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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S100 Calcium Binding Protein A1 (S100A1) ELISA Kit
S100A1 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A1 (S100A1) ELISA KitS100A1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A1 (S100A1) ELISA KitS100A1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A1 (S100A1) ELISA KitS100A1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A1 (S100A1) ELISA KitS100A1 适用: 人 Colorimetric Sandwich ELISA 0.82-200 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all S100A1 ELISA试剂盒
- S100A1 (S100 Calcium Binding Protein A1 (S100A1))
- 别名
- S100A1 (S100A1 产品)
- 别名
- S100 ELISA Kit, S100-alpha ELISA Kit, S100A ELISA Kit, S100A1 ELISA Kit, DKFZp468K0511 ELISA Kit, AI266795 ELISA Kit, S100a ELISA Kit, S100 calcium binding protein A1 ELISA Kit, S100A1 ELISA Kit, S100a1 ELISA Kit, s100a1 ELISA Kit
- UniProt
- P56565
- 途径
- Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins
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