Nicastrin ELISA 试剂盒
NCSTN
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all Nicastrin (NCSTN) ELISA试剂盒
- Nicastrin (NCSTN)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Nicastrin/NCSTN ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Nicastrin.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product NCSTN ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Nicastrin (NCSTN) ELISA试剂盒
- Nicastrin (NCSTN)
- 别名
- Nicastrin (NCSTN (NCSTN 产品)
- 别名
- CG7012 ELISA Kit, Dmel\\CG7012 ELISA Kit, Nct ELISA Kit, Nic ELISA Kit, S5A ELISA Kit, ST96B ELISA Kit, agro ELISA Kit, dNct ELISA Kit, nic ELISA Kit, NCSTN ELISA Kit, DKFZp469O2418 ELISA Kit, APH2 ELISA Kit, 9430068N19Rik ELISA Kit, AA727311 ELISA Kit, Aph2 ELISA Kit, D1Dau13e ELISA Kit, Kiaa0253 ELISA Kit, mKIAA0253 ELISA Kit, nicastrin ELISA Kit, Nicastrin ELISA Kit, NCSTN ELISA Kit, nct ELISA Kit, ncstn ELISA Kit, CpipJ_CPIJ001617 ELISA Kit, Ncstn ELISA Kit, aph-2 ELISA Kit
- UniProt
- P57716
- 途径
- Notch Signaling, Neurotrophin Signaling Pathway
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