LGMN ELISA 试剂盒
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- 抗原 See all LGMN ELISA试剂盒
- LGMN (Legumain (LGMN))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Legumain/Asparaginyl Endopeptidase ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Legumain.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product LGMN ELISA Kit
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Legumain (LGMN) ELISA Kit
LGMN 适用: Cow Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Legumain (LGMN) ELISA KitLGMN 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Legumain (LGMN) ELISA KitLGMN 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Legumain (LGMN) ELISA KitLGMN 适用: 小鼠 AA 18-435 Colorimetric Sandwich ELISA
Legumain (LGMN) ELISA KitLGMN 适用: 人 AA 18-433 Colorimetric Sandwich ELISA
Legumain (LGMN) ELISA KitLGMN 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Legumain (LGMN) ELISA KitLGMN 适用: 小鼠 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all LGMN ELISA试剂盒
- LGMN (Legumain (LGMN))
- 别名
- Legumain (Asparaginyl Endopeptidase (LGMN 产品)
- 别名
- AEP ELISA Kit, LGMN1 ELISA Kit, PRSC1 ELISA Kit, AI746452 ELISA Kit, AU022324 ELISA Kit, Prsc1 ELISA Kit, MGC64351 ELISA Kit, cb173 ELISA Kit, cb460 ELISA Kit, sb:cb173 ELISA Kit, zgc:76953 ELISA Kit, wu:fa98g05 ELISA Kit, wu:fj92g08 ELISA Kit, lgmn ELISA Kit, MGC88980 ELISA Kit, DELTA VACUOLAR PROCESSING ENZYME ELISA Kit, DELTAVPE ELISA Kit, delta vacuolar processing enzyme ELISA Kit, legumain ELISA Kit, legumain L homeolog ELISA Kit, delta vacuolar processing enzyme ELISA Kit, Legumain ELISA Kit, LGMN ELISA Kit, Lgmn ELISA Kit, lgmn.L ELISA Kit, lgmn ELISA Kit, DELTA-VPE ELISA Kit, MBOO_RS07785 ELISA Kit, Odosp_3582 ELISA Kit, LOC569153 ELISA Kit
- UniProt
- O89017
- 途径
- Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades
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