Ephrin A5 ELISA 试剂盒
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- 抗原 See all Ephrin A5 (EFNA5) ELISA试剂盒
- Ephrin A5 (EFNA5)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse EFNA5 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse EFNA5.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product EFNA5 ELISA Kit
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Ephrin A5 (EFNA5) ELISA Kit
EFNA5 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Ephrin A5 (EFNA5) ELISA KitEFNA5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Ephrin A5 (EFNA5) ELISA试剂盒
- Ephrin A5 (EFNA5)
- 别名
- EFNA5 (EFNA5 产品)
- 别名
- EFNA5 ELISA Kit, af1 ELISA Kit, efl5 ELISA Kit, rags ELISA Kit, eplg7 ELISA Kit, lerk7 ELISA Kit, AL-1 ELISA Kit, AV158822 ELISA Kit, EFL-5 ELISA Kit, Ephrin-A5 ELISA Kit, Epl7 ELISA Kit, LERK-7 ELISA Kit, RAGS ELISA Kit, Lerk7 ELISA Kit, AF1 ELISA Kit, EFL5 ELISA Kit, EPLG7 ELISA Kit, GLC1M ELISA Kit, LERK7 ELISA Kit, ephrin A5 ELISA Kit, EFNA5 ELISA Kit, efna5 ELISA Kit, Efna5 ELISA Kit
- UniProt
- O08543
- 途径
- RTK signaling
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