CSTB ELISA 试剂盒
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- 抗原 See all CSTB ELISA试剂盒
- CSTB (Cystatin B (Stefin B) (CSTB))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Cystatin B ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Cystatin B.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling CSTB ELISA Kit
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Cystatin B (Stefin B) (CSTB) ELISA Kit
CSTB 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin B (Stefin B) (CSTB) ELISA KitCSTB 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Cystatin B (Stefin B) (CSTB) ELISA KitCSTB 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin B (Stefin B) (CSTB) ELISA KitCSTB 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin B (Stefin B) (CSTB) ELISA KitCSTB 适用: 人 AA 2-98 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Cystatin B (Stefin B) (CSTB) ELISA KitCSTB 适用: 人 Colorimetric Sandwich ELISA 0.0938-6 ng/mL Cell Culture Cells, Cerebrospinal Fluid, Milk, Plasma, Saliva, Serum, Urine
Cystatin B (Stefin B) (CSTB) ELISA KitCSTB 适用: 人 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CSTB ELISA试剂盒
- CSTB (Cystatin B (Stefin B) (CSTB))
- 别名
- Cystatin B (CSTB 产品)
- 别名
- CST6 ELISA Kit, EPM1 ELISA Kit, EPM1A ELISA Kit, PME ELISA Kit, STFB ELISA Kit, ULD ELISA Kit, CSTB ELISA Kit, Cyb ELISA Kit, Epm1 ELISA Kit, Stfb ELISA Kit, MGC189005 ELISA Kit, pme ELISA Kit, cst6 ELISA Kit, epm1 ELISA Kit, stfb ELISA Kit, AA960480 ELISA Kit, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 ELISA Kit, ATCYS6 ELISA Kit, ATCYSB ELISA Kit, cystatin B ELISA Kit, LOC100008600 ELISA Kit, LOC100136235 ELISA Kit, CPI1 ELISA Kit, cystatin B ELISA Kit, cystatin B (stefin B) ELISA Kit, cystatin 14a, tandem duplicate 2 ELISA Kit, cystatin-B ELISA Kit, CSTB ELISA Kit, Cstb ELISA Kit, cstb ELISA Kit, cst14a.2 ELISA Kit, CYSB ELISA Kit, cpi1 ELISA Kit, LOC101123265 ELISA Kit
- UniProt
- Q62426
- 途径
- Response to Water Deprivation
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