CLIC4 ELISA 试剂盒
CLIC4
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all CLIC4 ELISA试剂盒
- CLIC4 (Chloride Intracellular Channel 4 (CLIC4))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human CLIC4 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human CLIC4.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product CLIC4 ELISA Kit
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Chloride Intracellular Channel 4 (CLIC4) ELISA Kit
CLIC4 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CLIC4 ELISA试剂盒
- CLIC4 (Chloride Intracellular Channel 4 (CLIC4))
- 别名
- CLIC4 (CLIC4 产品)
- 别名
- CLIC4L ELISA Kit, H1 ELISA Kit, MTCLIC ELISA Kit, huH1 ELISA Kit, p64H1 ELISA Kit, cb142 ELISA Kit, wu:fb33c05 ELISA Kit, wu:fc30h10 ELISA Kit, clic4 ELISA Kit, MGC89006 ELISA Kit, CLIC4 ELISA Kit, DKFZp459A0325 ELISA Kit, D0Jmb3 ELISA Kit, TU-74 ELISA Kit, mc3s5 ELISA Kit, mtCLIC ELISA Kit, xclic4 ELISA Kit, chloride intracellular channel 4 ELISA Kit, chloride intracellular channel 4 (mitochondrial) ELISA Kit, chloride intracellular channel 4 S homeolog ELISA Kit, CLIC4 ELISA Kit, Clic4 ELISA Kit, clic4 ELISA Kit, clic4.S ELISA Kit
- UniProt
- Q9Y696
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