CD166 ELISA 试剂盒
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- 抗原 See all CD166 (ALCAM) ELISA试剂盒
- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse ALCAM/CD166 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse ALCAM.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product ALCAM ELISA Kit
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Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit
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Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 兔 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 人 AA 28-526 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 小鼠 AA 28-527 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 大鼠 AA 28-527 Colorimetric Sandwich ELISA
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 小鼠 Colorimetric Sandwich ELISA 0.468 ng/mL - 30 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA KitALCAM 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CD166 (ALCAM) ELISA试剂盒
- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
- 别名
- ALCAM (CD166 (ALCAM 产品)
- 别名
- CD166 ELISA Kit, MEMD ELISA Kit, ALCAM ELISA Kit, BEN ELISA Kit, DM-GRASP ELISA Kit, JC7 ELISA Kit, SC1 ELISA Kit, AI853494 ELISA Kit, MuSC ELISA Kit, alcam ELISA Kit, cd166 ELISA Kit, memd ELISA Kit, fb53h09 ELISA Kit, fb80a10 ELISA Kit, nlcam ELISA Kit, si:ch211-190h2.4 ELISA Kit, wu:fb53h09 ELISA Kit, wu:fb80a10 ELISA Kit, wu:fc14b01 ELISA Kit, activated leukocyte cell adhesion molecule ELISA Kit, activated leukocyte cell adhesion molecule a ELISA Kit, activated leukocyte cell adhesion molecule L homeolog ELISA Kit, CD166 antigen homolog ELISA Kit, activated leukocyte cell adhesion molecule b ELISA Kit, ALCAM ELISA Kit, Alcam ELISA Kit, alcama ELISA Kit, alcam.L ELISA Kit, alcam ELISA Kit, cd166 ELISA Kit, alcamb ELISA Kit
- UniProt
- Q61490
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