Angiostatin ELISA 试剂盒
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- 抗原 See all Angiostatin (ANG) ELISA试剂盒
- Angiostatin (ANG)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0545
- Supplier
- Rockland
- 原理
- Human Angiostatin K1-3 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human Angiostatin K1-3. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product ANG ELISA Kit
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiostatin K1-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human serum, plasma or cell culture supernates to each empty well. It is recommended that each human Angiostatin K1-3 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: V98-S355(K1-3)
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all Angiostatin (ANG) ELISA试剂盒
- Angiostatin (ANG)
- 别名
- Angiostatin K1-3 (ANG 产品)
- 背景
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Synonyms: Ang K1-3
Background: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(ED₅₀ =70 nM) relative to kringles 1-4(ED₅₀ = 135 nM).
- 基因ID
- 5340
- NCBI登录号
- NP_000292
- UniProt
- P00747
- 途径
- Lipid Metabolism
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