Osteopontin ELISA 试剂盒
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- 抗原 See all Osteopontin (SPP1) ELISA试剂盒
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0287
- Supplier
- Rockland
- 原理
- Human OPN Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human OPN. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product SPP1 ELISA Kit
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Secreted phosphoprotein 1 (SPP1) ELISA Kit
SPP1 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 小鼠 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 犬 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 Colorimetric Sandwich ELISA 50-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 Colorimetric Sandwich ELISA Milk, Plasma (EDTA), Tissue Culture Medium, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 犬 Colorimetric Sandwich ELISA 0.3125 ng/mL - 10 ng/mL Plasma, Serum, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.06 ng/mL - 4 ng/mL Plasma, Serum, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human OPN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum, plasma(heparin, EDTA), milk or urine to each empty well. It is recommended that each human OPN standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: I17-N300
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all Osteopontin (SPP1) ELISA试剂盒
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- 别名
- OPN (SPP1 产品)
- 背景
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Synonyms: BNSP, Bone sialoprotein 1, Bone sialoprotein I, BSPI, Early T lymphocyte activation 1, ETA 1, MGC110940, Nephropontin, OPN, Osteopontin, OSTP_HUMAN, Secreted phosphoprotein 1, Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1), Secreted phosphoprotein 1 (osteopontin bone sialoprotein), SPP-1, SPP1, Urinary stone protein, Uropontin
Background: Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.
- 基因ID
- 6696
- NCBI登录号
- NP_000573
- UniProt
- P10451
- 途径
- Regulation of Cell Size
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