MMP7 ELISA 试剂盒
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- 抗原 See all MMP7 ELISA试剂盒
- MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0271
- Supplier
- Rockland
- 原理
- Human MMP-7 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human total MMP-7. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 6 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product MMP7 ELISA Kit
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin), saliva or urine to each empty well. It is recommended that each human MMP-7 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: L18-K267
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all MMP7 ELISA试剂盒
- MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
- 别名
- MMP-7 (MMP7 产品)
- 背景
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Synonyms: Matrilysin, Matrin, Matrix metalloproteinase 7, Matrix metalloproteinase-7, MMP-7, MMP7, MMP7_HUMAN, MPSL1, Pump 1 protease, Pump-1 protease, PUMP1, Uterine metalloproteinase
Background: Matrix metalloproteinase-7(MMP-7) previously called putative metalloproteinase I(PUMP1) or matrilysin. The PUMP1 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The PUMP I protein has 267 amino acids and is significantly shorter than stromelysin or collagenase(477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. Matrilysin, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, matrilysin mRNA is regulated in a specific and distinct manner in normal and malignant tissues. Matrilysin has been shown to correlate with nodal or distant metastasis in colorectal carcinomas, however, its implication in early invasive colorectal carcinomas has not been determined.1 Matrilysin is also a mediator of pulmonary fibrosis and a ...
- 基因ID
- 4316
- NCBI登录号
- NP_002414
- UniProt
- P09237
- 途径
- Production of Molecular Mediator of Immune Response
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