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MMP1 ELISA 试剂盒

Rockland koa0266 MMP1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
Rockland
产品编号 ABIN6720383
Supplier Product No.: koa0266
发货至: 中国
  • 抗原 See all MMP1 ELISA试剂盒
    MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
    适用
    • 20
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156 pg/mL - 10000 pg/mL
    最低检测浓度
    156 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0266
    Supplier
    Rockland
    原理
    Human MMP-1 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human total MMP-1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 10 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product MMP1 ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin) to each empty well. It is recommended that each human MMP-1 standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: NSO, Immunogen sequence:  F20-N469

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all MMP1 ELISA试剂盒
    MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
    别名
    MMP-1 (MMP1 产品)
    背景

    Synonyms: 27 kDa interstitial collagenase, CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metallopeptidase 1 (interstitial collagenase), Matrix Metalloproteinase 1, Matrix metalloproteinase-1, MMP 1, MMP-1, MMP1, MMP1_HUMAN

    Background: Matrix metalloproteinase-1(MMP-1) is also known as collagenase. Matrix metalloproteinases(MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1(MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1(interstitial collagenase), MMP9(gelatinase B) and MMP12(macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.

    基因ID
    4312
    NCBI登录号
    NP_001139410
    UniProt
    B4DN15
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