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EGF ELISA 试剂盒

EGF 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate, Urine
产品编号 ABIN6720371
发货至: 中国
  • 抗原 See all EGF ELISA试剂盒
    EGF (Epidermal Growth Factor (EGF))
    适用
    • 18
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    7.8 pg/mL - 500 pg/mL
    最低检测浓度
    7.8 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate, Urine
    Analytical Method
    Quantitative
    产品特性

    Synonyms: AI790464, Beta urogastrone, Epidermal growth factor, HOMG4, OTTHUMP00000219721, OTTHUMP00000219722, Pro epidermal growth factor, Pro epidermal growth factor precursor (EGF), URG, Urogastrone

    Background: Epidermal growth factor(EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

    Gene Name: EGF

    Production: Natural and recombinant rat EGF. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: N974-R1026

    Top Product
    Discover our top product EGF ELISA Kit
  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL rat EGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. It is recommended that each rat EGF standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 7.8pg/mL-500pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all EGF ELISA试剂盒
    EGF (Epidermal Growth Factor (EGF))
    别名
    EGF (EGF 产品)
    别名
    HOMG4 ELISA Kit, URG ELISA Kit, AI790464 ELISA Kit, CEGF ELISA Kit, epidermal growth factor ELISA Kit, pro-epidermal growth factor ELISA Kit, EGF ELISA Kit, egf ELISA Kit, CpipJ_CPIJ020278 ELISA Kit, Egf ELISA Kit
    基因ID
    25313
    NCBI登录号
    NP_036974
    UniProt
    P07522
    途径
    NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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