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MFGE8 ELISA 试剂盒

Rockland koa0698 MFGE8 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
Rockland
产品编号 ABIN6720367
Supplier Product No.: koa0698
发货至: 中国
  • 抗原 See all MFGE8 ELISA试剂盒
    MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
    适用
    • 8
    • 7
    • 3
    • 1
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5 pg/mL - 4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0698
    Supplier
    Rockland
    原理
    Human MFGE8 - Lactadherin Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human MFGE8. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 10 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product MFGE8 ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human MFGE8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. We recommend that each human MFGE8 standard solution and each sample is measured in duplicate.
    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: L24-C387

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all MFGE8 ELISA试剂盒
    MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
    别名
    MFGE8/Lactadherin (MFGE8 产品)
    背景

    Synonyms: BA46, Breast epithelial antigen BA46, Contains Medin, EDIL1, HMFG, hP47, HsT19888, Lactadherin, Lactadherin [Precursor], Medin, MFG E8, MFG-E8, MFGE8, MFGM, MFGM_HUMAN, Milk fat globule EGF factor 8, Milk fat globule EGF factor 8 protein, Milk fat globule-EGF factor 8, O acetyl disialoganglioside synthase, OAcGD3S, SED1, SPAG10, Sperm associated antigen 10, Sperm surface protein hP47

    Background: MFGE8(Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.

    基因ID
    4240
    NCBI登录号
    NP_001108086
    UniProt
    Q08431
    途径
    SARS-CoV-2 Protein Interactome
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