Amphiregulin ELISA 试剂盒
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- 抗原 See all Amphiregulin (AREG) ELISA试剂盒
- Amphiregulin (AREG)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0144
- Supplier
- Rockland
- 原理
- Human Amphiregulin (AR) Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human Amphiregulin. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product AREG ELISA Kit
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Amphiregulin (AREG) ELISA Kit
AREG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Amphiregulin (AREG) ELISA KitAREG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Amphiregulin (AREG) ELISA KitAREG 适用: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Amphiregulin (AREG) ELISA KitAREG 适用: 人 Colorimetric Sandwich ELISA 4.69 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Amphiregulin (AREG) ELISA KitAREG 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Amphiregulin (AREG) ELISA KitAREG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Amphiregulin (AREG) ELISA KitAREG 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
Amphiregulin (AREG) ELISA KitAREG 适用: 人 AA 101-198 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Amphiregulin (AREG) ELISA KitAREG 适用: 小鼠 AA 100-248 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Amphiregulin (AREG) ELISA KitAREG 适用: 兔 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Plasma, Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Amphiregulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. It is recommended that each human Amphiregulin standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: S101-K198
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all Amphiregulin (AREG) ELISA试剂盒
- Amphiregulin (AREG)
- 别名
- Amphiregulin(AR) (AREG 产品)
- 背景
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Synonyms: A REG, AR, AREG, Colorectum cell derived growth
Background: Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. Amphiregulin is related to epidermal growth factor(EGF) and transforming growth factor alpha(TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.
- 基因ID
- 374
- NCBI登录号
- NP_001648
- UniProt
- P15514
- 途径
- RTK signaling, EGFR Signaling Pathway
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