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PRSS8 ELISA 试剂盒

Rockland koa0749 PRSS8 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
Rockland
产品编号 ABIN6720348
Supplier Product No.: koa0749
发货至: 中国
  • 抗原 See all PRSS8 ELISA试剂盒
    PRSS8 (Protease, serine, 8 (PRSS8))
    适用
    • 13
    • 4
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.78 ng/mL - 50 ng/mL
    最低检测浓度
    0.78 ng/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0749
    Supplier
    Rockland
    原理
    Mouse Prostasin Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant mouse Prostasin. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 50 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Prostasin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. We recommend that each mouse Prostasin standard solution and each sample is measured in duplicate.
    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: A30-Q289

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all PRSS8 ELISA试剂盒
    PRSS8 (Protease, serine, 8 (PRSS8))
    别名
    Prostasin (PRSS8 产品)
    背景

    Synonyms: 2410039E18Rik, AI313909, C79772, CAP1, Channel Activating Protease 1, mCAP1, Prostasin, Prostasin heavy chain, protease, serine, 8, Prss8, PRSS8_HUMAN, Serine protease 9

    Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60 % to 80 % increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

    基因ID
    76560
    NCBI登录号
    NP_579929
    UniProt
    Q9ESD1
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