TGFA ELISA 试剂盒
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- 抗原 See all TGFA ELISA试剂盒
- TGFA (Transforming Growth Factor, alpha (TGFA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0306
- Supplier
- Rockland
- 原理
- Human TGF alpha Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Milk, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human TGF alpha. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 1 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product TGFA ELISA Kit
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Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitSmall Sample TGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: Cow Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 人 AA 40-89 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum
Transforming Growth Factor, alpha (TGFA) ELISA KitTGFA 适用: 人 Colorimetric Sandwich ELISA 3-1000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TGF alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or milk to each empty well. It is recommended that each human TGF alpha standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: V40-A89
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all TGFA ELISA试剂盒
- TGFA (Transforming Growth Factor, alpha (TGFA))
- 别名
- TGF alpha (TGFA 产品)
- 背景
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Synonyms: EGF like TGF, ETGF, OTTHUMP00000203300, OTTHUMP00000203301, OTTHUMP00000203345, OTTHUMP00000204192, Protransforming growth factor alpha, RATTGFAA, TFGA, TGF A, TGF type 1, TGFA, Transforming growth factor alpha, Transforming growth factor alpha precursor, Wa1, Waved 1
Background: Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to human chromosome 2 close to the breakpoint of the t(2,8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells, the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.
- 基因ID
- 7039
- NCBI登录号
- NP_001093161
- UniProt
- P01135
- 途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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