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IL-33 ELISA 试剂盒

IL33 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
产品编号 ABIN6720339
发货至: 中国
  • 抗原 See all IL-33 (IL33) ELISA试剂盒
    IL-33 (IL33) (Interleukin 33 (IL33))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6 pg/mL - 1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    产品特性

    Synonyms: C9orf26, DVS27, DVS27 related protein, IL-1F11, IL-33, IL1F11, IL33, IL33_HUMAN, Interleukin-1 family member 11, Interleukin-33, NF-HEV, NFEHEV, Nuclear factor from high endothelial venules, RP11-575C20.2

    Background: Interleukin 33(IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell(CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.

    Gene Name: IL33

    Production: Natural and recombinant human IL-33. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: S112-T270

    Top Product
    Discover our top product IL33 ELISA Kit
  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-33 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or cell lysates to each empty well. It is recommended that each human IL-33 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 15.6pg/mL-1000pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all IL-33 (IL33) ELISA试剂盒
    IL-33 (IL33) (Interleukin 33 (IL33))
    别名
    IL-33 (IL33 产品)
    别名
    IL33 ELISA Kit, C9orf26 ELISA Kit, DVS27 ELISA Kit, IL1F11 ELISA Kit, NF-HEV ELISA Kit, NFEHEV ELISA Kit, RP11-575C20.2 ELISA Kit, 9230117N10Rik ELISA Kit, Il-33 ELISA Kit, Il1f11 ELISA Kit, RGD1311155 ELISA Kit, interleukin 33 ELISA Kit, IL33 ELISA Kit, Il33 ELISA Kit
    基因ID
    90865
    NCBI登录号
    NP_001186569
    UniProt
    O95760
    途径
    Production of Molecular Mediator of Immune Response
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