CCL5 ELISA 试剂盒
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- 抗原 See all CCL5 ELISA试剂盒
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0293
- Supplier
- Rockland
- 原理
- Human Rantes Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human Rantes. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 3 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product CCL5 ELISA Kit
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Rantes standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human Rantes standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: S24-S91
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all CCL5 ELISA试剂盒
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
- 别名
- Rantes (CCL5 产品)
- 背景
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Synonyms: Beta chemokine RANTES, Beta chemokine RANTES precursor, C C motif chemokine 5, CCL5_HUMAN, Chemokine ligand 5, Chemokine CC Motif Ligand 5, EoCP, Eosinophil chemotactic cytokine, RANTES, Regulated upon activation normally T expressed and presumably secreted, SCYA5, SIS delta, SIS-delta, SISd, Small inducible cytokine A5, Small inducible cytokine subfamily A member 5, T cell specific protein p288, T cell specific protein RANTES, T-cell-specific protein RANTES, TCP 228, TCP228
Background: Rantes(regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
- 基因ID
- 6352
- NCBI登录号
- NP_002976
- UniProt
- D0EI67
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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