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AGRP ELISA 试剂盒

Rockland koa0862 AGRP 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
Rockland
产品编号 ABIN6720335
Supplier Product No.: koa0862
发货至: 中国
  • 抗原 See all AGRP ELISA试剂盒
    AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    7.8 pg/mL - 500 pg/mL
    最低检测浓度
    7.8 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0862
    Supplier
    Rockland
    原理
    Human AGRP - agouti-related Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human AGRP. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 4 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product AGRP ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human AGRP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human AGRP standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: sf21, Immunogen sequence: A21-T132

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all AGRP ELISA试剂盒
    AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))
    别名
    AGRP (AGRP 产品)
    背景

    Synonyms: Agrp, AGRT, ART, ASIP2

    Background: Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

    基因ID
    181
    NCBI登录号
    NP_001129
    UniProt
    O00253
    途径
    Feeding Behaviour, Photoperiodism
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