TGFB3 ELISA 试剂盒
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- 抗原 See all TGFB3 ELISA试剂盒
- TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0630
- Supplier
- Rockland
- 原理
- Rat TGF beta 3 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA), Serum, Urine
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant rat TGF-beta 3. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product TGFB3 ELISA Kit
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat TGF-beta 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated rat cell culture supernates, serum, plasma(EDTA) or urine to each empty well. It is recommended that each rat TGF-beta 3 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: sf21, Immunogen sequence: A301-S412
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all TGFB3 ELISA试剂盒
- TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))
- 别名
- TGF-beta 3 (TGFB3 产品)
- 背景
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Synonyms: ARVD, ARVD1, FLJ16571, MGC105479, MGC118722, prepro-transforming growth factor beta-3, RNHF, TGF beta 3, TGF beta3, TGF-beta-3, TGFB 3, TGFB3, TGFB3_HUMAN, transforming growth factor beta 3, Transforming growth factor beta-3
Background: Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4.
- 基因ID
- 25717
- NCBI登录号
- NP_037306
- UniProt
- Q07258
- 途径
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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