Granulin ELISA 试剂盒
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- 抗原 See all Granulin (GRN) ELISA试剂盒
- Granulin (GRN)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: Acrogranin, CLN11, GEP, GP88, Granulin A, Granulin B, Granulin C, Granulin D, Granulin E, Granulin epithelin, Granulin F, Granulin G, Granulin-7, Granulins, GRN, GRN_HUMAN, PC cell derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, Progranulin
Background: Granulin, also known as Proepithelin(PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
Gene Name: GRN
Production: Natural and recombinant human Progranulin. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: T18-L593
- Top Product
- Discover our top product GRN ELISA Kit
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Progranulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine or human milk to each empty well. We recommend that each human Progranulin standard solution and each sample is measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 1.56 ng/mL-100 ng/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- 抗原 See all Granulin (GRN) ELISA试剂盒
- Granulin (GRN)
- 别名
- Progranulin (GRN 产品)
- 别名
- GRN ELISA Kit, grn ELISA Kit, wu:fb11g12 ELISA Kit, wu:fj18f05 ELISA Kit, MGC53354 ELISA Kit, DKFZp459H066 ELISA Kit, CLN11 ELISA Kit, GEP ELISA Kit, GP88 ELISA Kit, PCDGF ELISA Kit, PEPI ELISA Kit, PGRN ELISA Kit, epithelin ELISA Kit, acrogranin ELISA Kit, granulin precursor ELISA Kit, granulin a ELISA Kit, Granulins ELISA Kit, granulin L homeolog ELISA Kit, granulin ELISA Kit, GRN ELISA Kit, grna ELISA Kit, grn ELISA Kit, grn.L ELISA Kit, granulin ELISA Kit, ORF1 granulin ELISA Kit, Grn ELISA Kit
- 物质类
- Viral Protein
- 基因ID
- 2896
- NCBI登录号
- NP_002078
- UniProt
- P28799
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