Aggrecan ELISA 试剂盒
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- 抗原 See all Aggrecan (ACAN) ELISA试剂盒
- Aggrecan (ACAN)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0548
- Supplier
- Rockland
- 原理
- Human Aggrecan Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Serum, Urine
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human Aggrecan. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 50 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product ACAN ELISA Kit
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Aggrecan (ACAN) ELISA Kit
ACAN 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Aggrecan (ACAN) ELISA KitACAN 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Aggrecan (ACAN) ELISA KitACAN 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Aggrecan (ACAN) ELISA KitACAN 适用: 兔 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Aggrecan (ACAN) ELISA KitACAN 适用: 小鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Aggrecan (ACAN) ELISA KitACAN 适用: 兔 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Aggrecan (ACAN) ELISA KitACAN 适用: 人 AA 20-675 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Serum, Urine
Aggrecan (ACAN) ELISA KitACAN 适用: Cow Colorimetric Competition ELISA 5.0-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Aggrecan (ACAN) ELISA KitACAN 适用: 兔 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Aggrecan standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or urine to each empty well. It is recommended that each human Aggrecan standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: V20-G675
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all Aggrecan (ACAN) ELISA试剂盒
- Aggrecan (ACAN)
- 别名
- Aggrecan (ACAN 产品)
- 背景
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Synonyms: ACAN, AGC1, AGCAN, Aggrecan 1, Aggrecan core protein, Aggrecan proteoglycan, Aggrecan structural proteoglycan of cartilage, Aggrecan1, ATEGQV, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122, Chondroitin sulfate proteoglycan core protein 1, CSPG1, CSPGCP, JSCATE, Large aggregating proteoglycan, mcspg, mgsk16, MSK16, SEDK
Background: Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.
- 基因ID
- 176
- UniProt
- P16112
- 途径
- Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport
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