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MMP12 ELISA 试剂盒

Rockland koa0622 MMP12 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Rockland
产品编号 ABIN6720310
Supplier Product No.: koa0622
发货至: 中国
  • 抗原 See all MMP12 ELISA试剂盒
    MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))
    适用
    • 12
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    62.5 pg/mL - 4000 pg/mL
    最低检测浓度
    62.5 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0622
    Supplier
    Rockland
    原理
    Mouse MMP-12 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (heparin), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant mouse MMP-12. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 10 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product MMP12 ELISA Kit
  • 应用备注

    ELISA_Dilution: 62.5pg/mL-4000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse MMP-12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each mouse MMP-12 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: A18-C462

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all MMP12 ELISA试剂盒
    MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))
    别名
    MMP-12 (MMP12 产品)
    背景

    Synonyms: EC 3.4.24.65, HME, Macrophage elastase, Macrophage metalloelastase, Macrophage metaloelastase, Matrix metallopeptidase 12 (macrophage elastase), Matrix metalloprotease 12, Matrix metalloproteinase-12, MGC138506, MME, MMP 12, MMP-12, Mmp12, MMP12_HUMAN

    Background: Matrix metalloproteinase-12(MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.

    基因ID
    17381
    NCBI登录号
    NP_001307005
    UniProt
    P34960
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