TXN ELISA 试剂盒
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- 抗原 See all TXN ELISA试剂盒
- TXN (Thioredoxin (TXN))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0739
- Supplier
- Rockland
- 原理
- Human Thioredoxin Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human Thioredoxin. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product TXN ELISA Kit
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- 应用备注
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ELISA_Dilution: 156pg/mL-10,000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Thioredoxin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Thioredoxin standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: V2-V105
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all TXN ELISA试剂盒
- TXN (Thioredoxin (TXN))
- 别名
- Thioredoxin (TXN 产品)
- 背景
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Synonyms: ADF, ATL-derived factor, DKFZp686B1993, MGC61975, SASP, Surface associated sulphydryl protein, Surface-associated sulphydryl protein, THIO_HUMAN, Thioredoxin, TRDX, TRX 1, Trx, TRX1, TXN, TXN protein
Background: Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. They can also act as electron donors to peroxidases and ribonucleotide reductase. Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It plays a role in many important biological processes, including redox signaling. This gene also plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). VDUP1 is a key stress-responsive inhibitor of Thioredoxin activity in cardiomyocytes.
- 基因ID
- 7295
- NCBI登录号
- NP_001231867
- UniProt
- P10599
- 途径
- Carbohydrate Homeostasis, Cell RedoxHomeostasis
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