FCN3 ELISA 试剂盒
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- 抗原 See all FCN3 ELISA试剂盒
- FCN3 (Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: Collagen/fibrinogen domain containing lectin 3 p35, Collagen/fibrinogen domain containing protein 3, FCN 3, FCNH, Ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen), Ficolin (collagen/fibrinogen domain containing) 3, Ficolin 3, H ficolin, HAKA1, Hakata antigen, MGC22543
Background: Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin(FN1), elastin(ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.
Gene Name: FCN3
Production: Natural and recombinant human Ficolin-3. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: Q24-R299
- Top Product
- Discover our top product FCN3 ELISA Kit
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Ficolin-3 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 0.78 ng/mL-50 ng/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- 抗原 See all FCN3 ELISA试剂盒
- FCN3 (Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3))
- 别名
- Ficolin-3 (FCN3 产品)
- 别名
- FCNH ELISA Kit, HAKA1 ELISA Kit, ficolin 3 ELISA Kit, ficolin-3 ELISA Kit, FCN3 ELISA Kit, Fcn3 ELISA Kit, fcn3-A ELISA Kit
- 基因ID
- 8547
- NCBI登录号
- NP_003656
- UniProt
- O75636
- 途径
- Complement System
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