FCN3 ELISA 试剂盒
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- 抗原 See all FCN3 ELISA试剂盒
- FCN3 (Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0743
- Supplier
- Rockland
- 原理
- Human Ficolin-3 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human Ficolin-3. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 20 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product FCN3 ELISA Kit
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Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA Kit
FCN3 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA KitFCN3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA KitFCN3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA KitFCN3 适用: 人 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA KitFCN3 适用: 人 AA 24-299 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (heparin), Serum
Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) ELISA KitFCN3 适用: 人 Colorimetric Sandwich ELISA 780-50000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Ficolin-3 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: Q24-R299
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all FCN3 ELISA试剂盒
- FCN3 (Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3))
- 别名
- Ficolin-3 (FCN3 产品)
- 背景
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Synonyms: Collagen/fibrinogen domain containing lectin 3 p35, Collagen/fibrinogen domain containing protein 3, FCN 3, FCNH, Ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen), Ficolin (collagen/fibrinogen domain containing) 3, Ficolin 3, H ficolin, HAKA1, Hakata antigen, MGC22543
Background: Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin(FN1), elastin(ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.
- 基因ID
- 8547
- NCBI登录号
- NP_003656
- UniProt
- O75636
- 途径
- Complement System
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