Neuropilin 1 ELISA 试剂盒
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- 抗原 See all Neuropilin 1 (NRP1) ELISA试剂盒
- Neuropilin 1 (NRP1)
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 780 pg/mL - 50000 pg/mL
- 最低检测浓度
- 780 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0808
- Supplier
- Rockland
- 原理
- Rat Neuropilin-1 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant rat Neuropilin-1. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 20 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product NRP1 ELISA Kit
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Neuropilin 1 (NRP1) ELISA Kit
NRP1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 大鼠 AA 22-854 Colorimetric Sandwich ELISA 780-50.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 人 AA 22-644 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 小鼠 AA 22-856 Colorimetric Sandwich ELISA 780-50.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Neuropilin 1 (NRP1) ELISA KitNRP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL rat Neuropilin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each rat Neuropilin-1 standard solution and each sample is measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: F22-D854
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all Neuropilin 1 (NRP1) ELISA试剂盒
- Neuropilin 1 (NRP1)
- 别名
- Neuropilin-1 (NRP1 产品)
- 背景
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Synonyms: A5 protein, BDCA4, BLOOD DENDRITIC CELL ANTIGEN 4, CD 304, CD304, DKFZp686A03134, DKFZp781F1414, Neuropilin-1, Neuropilin1, Neuropilin1 transmembrane receptor, NP1, NPN1, NRP 1, NRP, NRP1, NRP1_HUMAN, Vascular endothelial cell growth factor 165 receptor, VEGF165R
Background: NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.
- 基因ID
- 246331
- NCBI登录号
- NP_659566
- UniProt
- Q9QWJ9
- 途径
- Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals
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