TDGF1 ELISA 试剂盒
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- 抗原 See all TDGF1 ELISA试剂盒
- TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5 pg/mL - 4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0848
- Supplier
- Rockland
- 原理
- Mouse Cripto - TDGF1 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant mouse Cripto. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Featured
- Discover our best selling TDGF1 ELISA Kit
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Cripto standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Cripto standard solution and each sample is measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: R26-Q150
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all TDGF1 ELISA试剂盒
- TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
- 别名
- Cripto/TDGF1 (TDGF1 产品)
- 背景
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Synonyms: CR, CRGF, Cripto 1, Cripto 1 growth factor, cripto, Cripto-1 growth factor, Cripto1, Cripto1 growth factor, Epidermal growth factor like cripto protein CR1, Epidermal growth factor-like cripto protein CR1, TDGF 1, TDGF1, TDGF1_HUMAN, Teratocarcinoma derived growth factor 1, Teratocarcinoma-derived growth factor 1
Background: Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.
- 基因ID
- 21667
- NCBI登录号
- NP_033545
- UniProt
- P51865
- 途径
- EGFR Signaling Pathway
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