IL-25 ELISA 试剂盒
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- 抗原 See all IL-25 (IL25) ELISA试剂盒
- IL-25 (IL25) (Interleukin 25 (IL25))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5 pg/mL - 4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: IL 17E, IL 25, IL-17E, IL-25, IL17e, IL25, IL25_HUMAN, Interleukin 17E, Interleukin 25, Interleukin-17E, Interleukin-25, Interleukin17E, Interleukin25, OTTHUMP00000027946, OTTHUMP00000246238, UNQ3120/PRO10272
Background: Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-kB activation, and stimulate the production of IL8. What's more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.
Gene Name: IL25
Production: Natural and recombinant human IL-17E. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: Y33-G177
- Top Product
- Discover our top product IL25 ELISA Kit
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IL-17E standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human IL-17E standard solution and each sample is measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 62.5pg/mL-4000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- 抗原 See all IL-25 (IL25) ELISA试剂盒
- IL-25 (IL25) (Interleukin 25 (IL25))
- 别名
- IL-17E - IL-25 (IL25 产品)
- 别名
- IL17E ELISA Kit, IL-17e ELISA Kit, Il17e ELISA Kit, IL25 ELISA Kit, RGD1561632 ELISA Kit, interleukin 25 ELISA Kit, IL25 ELISA Kit, Il25 ELISA Kit
- 基因ID
- 64806
- NCBI登录号
- NP_073626
- UniProt
- Q9H293
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