2B4 ELISA 试剂盒
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- 抗原 See all 2B4 (CD244) ELISA试剂盒
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5 pg/mL - 4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: 2B4, C9.1, CD244, CD244 antigen, CD244 Molecule natural killer cell receptor 2B4, CD244 natural killer cell receptor 2B4, CD244_HUMAN, h2B4, Ly90, Natural killer cell activation-inducing ligand, Natural killer cell receptor 2B4, NK cell activation inducing ligand, NK cell activation inducing ligand NAIL, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, NKR2B4, Nmrk, Non-MHC restricted killing associated, p38, signaling lymphocytic activation molecule 4, SLAM family member 4, SLAMF4
Background: CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.
Gene Name: CD244
Production: Natural and recombinant human CD244. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: C22-R221
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CD244 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD244 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 62.5pg/mL-4000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all 2B4 (CD244) ELISA试剂盒
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
- 别名
- CD244 - 2B4 (CD244 产品)
- 别名
- CD244 ELISA Kit, 2B4 ELISA Kit, NAIL ELISA Kit, NKR2B4 ELISA Kit, Nmrk ELISA Kit, SLAMF4 ELISA Kit, C9.1 ELISA Kit, F730046O15Rik ELISA Kit, Ly90 ELISA Kit, CD244 molecule ELISA Kit, CD244 natural killer cell receptor 2B4 ELISA Kit, CD244 ELISA Kit, Cd244 ELISA Kit
- 基因ID
- 51744
- NCBI登录号
- NP_001160135
- UniProt
- Q9BZW8
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