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CD70 ELISA 试剂盒

Rockland koa0784 CD70 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
产品编号 ABIN6720189
Supplier Product No.: koa0784
发货至: 中国
  • 抗原 See all CD70 ELISA试剂盒
    CD70 (CD70 Molecule (CD70))
    适用
    • 6
    • 4
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    31.2 pg/mL - 2000 pg/mL
    最低检测浓度
    31.2 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0784
    Supplier
    Rockland
    原理
    Mouse CD70 - TNFSF7 - cd27L Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant mouse CD70. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 10 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CD70 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD70 standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: S45-P195

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all CD70 ELISA试剂盒
    CD70 (CD70 Molecule (CD70))
    别名
    CD70/TNFSF7/cd27L (CD70 产品)
    背景

    Synonyms: CD 27L, CD 70, CD27 L, CD27 LG, CD27 ligand, CD27-L, CD27L, CD27LG, CD70, CD70 antigen, CD70 Molecule, CD70_HUMAN, Ki 24 antigen, Ki24 antigen, Surface antigen CD70, TNFSF 7, TNFSF7, Tumor necrosis factor (ligand) superfamily member 7, Tumor necrosis factor ligand superfamily member

    Background: CD70(Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24 % amino acid sequence identity in the extracellular region.

    基因ID
    21948
    NCBI登录号
    NP_035747
    UniProt
    O55237
    途径
    Cancer Immune Checkpoints
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