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Ccl12 ELISA 试剂盒

Rockland koa0646 Ccl12 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
产品编号 ABIN6720185
Supplier Product No.: koa0646
发货至: 中国
  • 抗原 See all Ccl12 ELISA试剂盒
    Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    31.2 pg/mL - 2000 pg/mL
    最低检测浓度
    31.2 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0646
    Supplier
    Rockland
    原理
    Mouse CCL12 - MCP5 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant mouse CCL12. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 10 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product Ccl12 ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CCL12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CCL12 standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: G23-G104

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all Ccl12 ELISA试剂盒
    Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
    别名
    CCL12/MCP5 (Ccl12 产品)
    背景

    Synonyms: C C motif chemokine 12, Ccl12, MCP 1 related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Scya12, Small inducible cytokine A12

    Background: Chemokine(C-C motif) ligand 12(CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5(MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11.

    基因ID
    105242482
    NCBI登录号
    NP_035461
    UniProt
    Q62401
    途径
    Cellular Response to Molecule of Bacterial Origin
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