Ccl12 ELISA 试剂盒
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- 抗原 See all Ccl12 ELISA试剂盒
- Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: C C motif chemokine 12, Ccl12, MCP 1 related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Scya12, Small inducible cytokine A12
Background: Chemokine(C-C motif) ligand 12(CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5(MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11.
Gene Name: Ccl12
Production: Natural and recombinant mouse CCL12. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: G23-G104
- Top Product
- Discover our top product Ccl12 ELISA Kit
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CCL12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CCL12 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 31.2pg/mL-2000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- 抗原 See all Ccl12 ELISA试剂盒
- Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
- 别名
- CCL12 - MCP5 (Ccl12 产品)
- 别名
- MCP-5 ELISA Kit, Scya12 ELISA Kit, chemokine (C-C motif) ligand 12 ELISA Kit, Ccl12 ELISA Kit
- 基因ID
- 105242482
- NCBI登录号
- NP_035461
- UniProt
- Q62401
- 途径
- Cellular Response to Molecule of Bacterial Origin
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