Stanniocalcin 1 ELISA 试剂盒
-
- 抗原 See all Stanniocalcin 1 (STC1) ELISA试剂盒
- Stanniocalcin 1 (STC1)
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0847
- Supplier
- Rockland
- 原理
- Human Stanniocalcin 1 - STC1 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
-
Production: Natural and recombinant human STC1. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product STC1 ELISA Kit
-
Stanniocalcin 1 (STC1) ELISA Kit
STC1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Stanniocalcin 1 (STC1) ELISA KitSTC1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Stanniocalcin 1 (STC1) ELISA KitSTC1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Stanniocalcin 1 (STC1) ELISA KitSTC1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Stanniocalcin 1 (STC1) ELISA KitSTC1 适用: 人 Colorimetric Sandwich ELISA 1.88-120 ng/mL Plasma, Serum, Tissue Homogenate
Stanniocalcin 1 (STC1) ELISA KitSTC1 适用: 人 AA 34-247 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Stanniocalcin 1 (STC1) ELISA KitSTC1 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Stanniocalcin 1 (STC1) ELISA KitSTC1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human STC1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. It is recommended that each human STC1 standard solution and each sample be measured in duplicate.
- 说明
-
Standard: Expression system for standard: NSO, Immunogen sequence: V34-A247
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
-
- 抗原 See all Stanniocalcin 1 (STC1) ELISA试剂盒
- Stanniocalcin 1 (STC1)
- 别名
- Stanniocalcin 1/STC1 (STC1 产品)
- 背景
-
Synonyms: Stanniocalcin, Stanniocalcin-1, STC, STC-1, STC1, STC1_HUMAN
Background: Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.
- 基因ID
- 6781
- NCBI登录号
- NP_003146
- UniProt
- P52823
- 途径
- Hormone Activity
-
