PI3 ELISA 试剂盒
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- 抗原 See all PI3 ELISA试剂盒
- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0637
- Supplier
- Rockland
- 原理
- Human Elafin - PI3 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human Elafin. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product PI3 ELISA Kit
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Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA Kit
PI3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA KitPI3 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA KitPI3 适用: 人 AA 23-117 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA KitPI3 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Peptidase Inhibitor 3, Skin-Derived (PI3) ELISA KitPI3 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Elafin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human Elafin standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: NSO, Immunogen sequence: A23-Q117
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all PI3 ELISA试剂盒
- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
- 别名
- Elafin/PI3 (PI3 产品)
- 背景
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Synonyms: Cementoin, Elafin, Elastase specific inhibitor, ESI, MGC13613, Peptidase inhibitor 3, Peptidase inhibitor 3 skin derived, PI 3, PI3, Pre elafin, Protease inhibitor WAP3, SKALP, Skin derived antileukoproteinase, Trappin 2, WAP four disulfide core domain 14, WAP four disulfide core domain protein 14, WAP3, WFDC14
Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase(SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core(WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
- 基因ID
- 5266
- NCBI登录号
- NP_002629
- UniProt
- P19957
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