DIABLO ELISA 试剂盒
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- 抗原 See all DIABLO ELISA试剂盒
- DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0503
- Supplier
- Rockland
- 原理
- Human Diablo - SMAC Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human Diablo. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product DIABLO ELISA Kit
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Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA Kit
DIABLO 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA KitDIABLO 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA KitDIABLO 适用: 人 Colorimetric Sandwich ELISA 0.1-30 ng/mL Cell Culture Supernatant, Plasma, Serum
Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA KitDIABLO 适用: 人 AA 56-239 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA KitDIABLO 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Diablo standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Diablo standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: A56-D239
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all DIABLO ELISA试剂盒
- DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
- 别名
- Diablo/SMAC (DIABLO 产品)
- 背景
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Synonyms: DFNA64, DIABLO, diablo homolog, mitochondrial, diablo, IAP-binding mitochondrial protein, DIABLO-S, direct IAP-binding protein with low pI, mitochondrial Smac protein, second mitochondria-derived activator of caspase, SMAC3
Background: Diablo homolog, mitochondrial, also known as second mitochondria-derived activator of caspases(SMAC), is a protein that in humans is encoded by the DIABLO gene(direct IAP binding protein with low pI). It is mapped to 12q24.31. DIABLO can bind mammalian IAP homolog A(MIHA, or API3) and can also interact with MIHB, MIHC, and OpIAP, the baculoviral IAP. This gene encodes an inhibitor of apoptosis protein(IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Over expression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64.
- 基因ID
- 56616
- NCBI登录号
- NP_001265231
- UniProt
- Q9NR28
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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