CD73 ELISA 试剂盒
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- 抗原 See all CD73 (NT5E) ELISA试剂盒
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0859
- Supplier
- Rockland
- 原理
- Human CD73 - NT5E Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human CD73. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product NT5E ELISA Kit
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5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA Kit
NT5E 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 小鼠 Colorimetric Sandwich ELISA 0.125 mU/mL - 8 mU/mL Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 人 AA 27-547 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 小鼠 Colorimetric Sandwich ELISA 0.125-8 mU/mL Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 人 Colorimetric Sandwich ELISA 3.12-200 ng/mL Plasma, Serum, Tissue Homogenate
5'-Nucleotidase, Ecto (CD73) (NT5E) ELISA KitNT5E 适用: 大鼠 Colorimetric Sandwich ELISA 0.625-40 mIU/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CD73 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CD73 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: CHO, Immunogen sequence: W27-K547
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all CD73 (NT5E) ELISA试剂盒
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- 别名
- CD73/NT5E (NT5E 产品)
- 背景
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Synonyms: 5' NT, 5' nucleotidase (CD73)|5' nucleotidase precursor, 5' nucleotidase ecto, 5' nucleotidase ecto (CD73), 5'-NT, 5'-nucleotidase, 5NTD_HUMAN, CD73, CD73 antigen, E5NT, Ecto 5' nucleotidase, Ecto-5'-nucleotidase, eN, eNT, NT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase
Background: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
- 基因ID
- 4907
- NCBI登录号
- NP_001191742
- UniProt
- P21589
- 途径
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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