S100A8 ELISA 试剂盒
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- 抗原 See all S100A8 ELISA试剂盒
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0661
- Supplier
- Rockland
- 原理
- Mouse S100A8 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant mouse S100A8. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product S100A8 ELISA Kit
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S100 Calcium Binding Protein A8 (S100A8) ELISA Kit
S100A8 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: Cow Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: 人 AA 2-93 Colorimetric Sandwich ELISA
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: 小鼠 AA 2-89 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
S100 Calcium Binding Protein A8 (S100A8) ELISA KitS100A8 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
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ELISA_Dilution: 31.2 pg/mL-2000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse S100A8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse S100A8 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: P2-E89
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all S100A8 ELISA试剂盒
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
- 别名
- S100A8 (S100A8 产品)
- 背景
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Synonyms: 60B8AG, CAGA, Calgranulin-A, Calprotectin L1L subunit, CFAG, CGLA, CP-10, Cystic fibrosis antigen, L1Ag, Leukocyte L1 complex light chain, MA387, MIF, Migration inhibitory factor-related protein 8, MRP 8, MRP-8, MRP8, Myeloid-related protein 8, NIF, p8, Protein S100-A8, S100 calcium binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100 calcium-binding protein A8, S100A8, S10A8_HUMAN, Urinary stone protein band A
Background: S100 calcium-binding protein A8 (S100A8), also known as calgranulin A, is a protein that in humans is encoded by the S100A8 gene. It is mapped to chromosome 1q21.3 based on an alignment of the S100A8 sequence with the genomic sequence. The proteins S100A8 and S100A9 form a heterodimer called calprotectin which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. This gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
- 基因ID
- 20201
- NCBI登录号
- NP_038678
- UniProt
- P27005
- 途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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