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Endothelin ELISA 试剂盒

Rockland koa0579 适用: 人 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
产品编号 ABIN6720144
Supplier Product No.: koa0579
发货至: 中国
  • 抗原 See all Endothelin ELISA试剂盒
    Endothelin
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    3.9 pg/mL - 250 pg/mL
    最低检测浓度
    3.9 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0579
    Supplier
    Rockland
    原理
    Human Endothelin Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant human Endothelin. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 0.5 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 16.5pg/mL, 7.8pg/mL, 3.9pg/mL human Endothelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Endothelin standard solution and each sample is measured in duplicate.
    说明

    Standard: Expression system for standard: human peptide, Immunogen sequence: C53-W73

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all Endothelin ELISA试剂盒
    Endothelin
    Abstract
    Endothelin 产品
    背景

    Synonyms: Big endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1, EDN2, EDN2_HUMAN, Endothelin 2, Endothelin-2, ET 2, ET-2, ET2, PPET2, Preproendothelin 2, Preproendothelin-2, EDN3, EDN3_HUMAN, Endothelin-3, ET 3, ET-3, PPET3, Preproendothelin 3, Preproendothelin-3

    Background: The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.

    基因ID
    1906
    NCBI登录号
    NP_001161791
    UniProt
    P05305
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