ESM1 ELISA 试剂盒
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- 抗原 See all ESM1 ELISA试剂盒
- ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 23.4 pg/mL - 1500 pg/mL
- 最低检测浓度
- 23.4 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: Endothelial cell-specific molecule 1, ESM-1, Esm1, ESM1_HUMAN
Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2(fibroblast growth factor 2).
Gene Name: ESM1
Production: Natural and recombinant mouse ESM1. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: W20-R184
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/mL, 46.8pg/mL, 23.4pg/mL mouse ESM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse ESM1 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 23.4pg/mL-1500pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- 抗原 See all ESM1 ELISA试剂盒
- ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
- 别名
- ESM1 - Endocan (ESM1 产品)
- 别名
- ESM1 ELISA Kit, zgc:153027 ELISA Kit, 0610042H23Rik ELISA Kit, AV004503 ELISA Kit, ESM-1 ELISA Kit, Pg25 ELISA Kit, endocan ELISA Kit, endothelial cell specific molecule 1 ELISA Kit, endothelial cell-specific molecule 1 ELISA Kit, ESM1 ELISA Kit, esm1 ELISA Kit, Esm1 ELISA Kit
- 基因ID
- 71690
- NCBI登录号
- NP_076101
- UniProt
- Q9QYY7
- 途径
- Growth Factor Binding
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