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LGALS1/Galectin 1 ELISA 试剂盒

Rockland koa0449 LGALS1 适用: 小鼠 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
产品编号 ABIN6720130
Supplier Product No.: koa0449
发货至: 中国
  • 抗原 See all LGALS1/Galectin 1 (LGALS1) ELISA试剂盒
    LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
    适用
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    • 13
    • 6
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156 pg/mL - 10000 pg/mL
    最低检测浓度
    156 pg/mL
    应用范围
    ELISA
    Supplier Product No.
    koa0449
    Supplier
    Rockland
    原理
    Mouse Galectin-1 Sandwich ELISA Kit for Quantitative Detection
    品牌
    AccuSignal™
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    特异性

    Production: Natural and recombinant mouse Galectin-1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度
    < 5 pg/mL
    组件
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product LGALS1 ELISA Kit
  • 应用备注
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Galectin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Galectin-1 standard solution and each sample be measured in duplicate.
    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: A2-E135

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原 See all LGALS1/Galectin 1 (LGALS1) ELISA试剂盒
    LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
    别名
    Galectin-1 (LGALS1 产品)
    背景

    Synonyms: 14 kDa laminin-binding protein, 14 kDa lectin, Beta galactoside binding protein 14 kDa, Beta-galactoside-binding lectin L-14-I, Gal-1, GAL1, Galaptin, Galectin-1, GBP, HBL, HLBP14, HPL Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, LEG1_HUMAN, LGALS1, Putative MAPK-activating protein PM12, S-Lac lectin 1

    Background: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation. Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse. They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.

    基因ID
    16852
    NCBI登录号
    NP_032521
    UniProt
    P16045
    途径
    Carbohydrate Homeostasis
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