CD14 ELISA 试剂盒
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- 抗原 See all CD14 ELISA试剂盒
- CD14
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: CD 14, CD14 antigen, CD14 Molecule, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
Background: CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein(mCD14) and a monocyte or liver-derived soluble serum protein(sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor(along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide(LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein(LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
Gene Name: CD14
Production: Natural and recombinant mouse CD14. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: A18-P345
- Top Product
- Discover our top product CD14 ELISA Kit
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CD14 (CD14) ELISA Kit
CD14 适用: 人 Colorimetric Sandwich ELISA 0.234-15 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
CD14 (CD14) ELISA KitCD14 适用: 人 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Cell Lysate, Plasma, Serum
CD14 (CD14) ELISA KitCD14 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD14 (CD14) ELISA KitCD14 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
CD14 (CD14) ELISA KitCD14 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
CD14 (CD14) ELISA KitCD14 适用: 猴 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD14 (CD14) ELISA KitCD14 适用: Pig Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
CD14 (CD14) ELISA KitCD14 适用: Cow Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
CD14 (CD14) ELISA KitCD14 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
CD14 (CD14) ELISA KitCD14 适用: Pig Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CD14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD14 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all CD14 ELISA试剂盒
- CD14
- 别名
- CD14 (CD14 产品)
- 别名
- CD14 molecule ELISA Kit, CD14 antigen ELISA Kit, CD14 ELISA Kit, Cd14 ELISA Kit
- 基因ID
- 12475
- NCBI登录号
- NP_033971
- UniProt
- P10810
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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