Cardiotrophin 1 ELISA 试剂盒
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- 抗原 See all Cardiotrophin 1 (CTF1) ELISA试剂盒
- Cardiotrophin 1 (CTF1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: Cardiophin 1, Cardiophin1, Cardiotrophin I, Cardiotrophin-1, CardiotrophinI, CT 1, CT-1, CT1, CTF 1, Ctf1, CTF1_HUMAN
Background: Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80 % amino acid identity with the 203-amino acid mouse CT-1 sequence, however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidn...
Gene Name: CTF1
Production: Natural and recombinant human CT-1. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: S2-A201
- Top Product
- Discover our top product CTF1 ELISA Kit
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CT-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CT-1 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- 抗原 See all Cardiotrophin 1 (CTF1) ELISA试剂盒
- Cardiotrophin 1 (CTF1)
- 别名
- Cardiotrophin-1 (CTF1 产品)
- 别名
- CTF1 ELISA Kit, ct1 ELISA Kit, ct-1 ELISA Kit, ctf2 ELISA Kit, CT-1 ELISA Kit, CT1 ELISA Kit, cardiotrophin 1 ELISA Kit, cardiotrophin-1 ELISA Kit, CTF1 ELISA Kit, ctf1 ELISA Kit, Ctf1 ELISA Kit, LOC100736818 ELISA Kit
- 基因ID
- 1489
- NCBI登录号
- NP_001136016
- UniProt
- Q16619
- 途径
- JAK/STAT Signaling
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