Cardiotrophin 1 ELISA 试剂盒
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- 抗原 See all Cardiotrophin 1 (CTF1) ELISA试剂盒
- Cardiotrophin 1 (CTF1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0342
- Supplier
- Rockland
- 原理
- Human Cardiotrophin-1 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human CT-1. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 10 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product CTF1 ELISA Kit
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Cardiotrophin 1 (CTF1) ELISA Kit
CTF1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 Colorimetric Sandwich ELISA 0.2-60 ng/mL Cell Culture Supernatant, Plasma, Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 人 AA 2-201 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 AA 2-203 Colorimetric Sandwich ELISA
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CT-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CT-1 standard solution and each sample be measured in duplicate.
- 说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: S2-A201
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all Cardiotrophin 1 (CTF1) ELISA试剂盒
- Cardiotrophin 1 (CTF1)
- 别名
- Cardiotrophin-1 (CTF1 产品)
- 背景
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Synonyms: Cardiophin 1, Cardiophin1, Cardiotrophin I, Cardiotrophin-1, CardiotrophinI, CT 1, CT-1, CT1, CTF 1, Ctf1, CTF1_HUMAN
Background: Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80 % amino acid identity with the 203-amino acid mouse CT-1 sequence, however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidn...
- 基因ID
- 1489
- NCBI登录号
- NP_001136016
- UniProt
- Q16619
- 途径
- JAK/STAT Signaling
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